Wednesday, April 18, 2007
Multiple Egg Sacs
In breeding the females, I have noticed that they put out multiple egg sacs without having remated. They seem to be able to store sperm for long periods of time after mating with a single male. I am interested to see what sort of role this multiple egg sac laying will play in my manipulation. Will one of the male's sperm be used preferentially over another's? After that male's sperm is used up, will she then use the other's? Is the female able to influence the sperm used to fertilize eggs or does she use whatever is in her storage sac? I should be able to tell when I am able to see whether the percent of eggs hatched changes with each egg sac for the females mated with a sterile male and a fertile male. Maybe the mate order will play a role. This could be pretty interesting! I think I would use MANOVA here?
Subscribe to:
Post Comments (Atom)
2 comments:
Hey Carmella - I'm not sure what your independent and response variables are here, but MANOVA is used when you have multiple response variables. You could do multiple ANOVAs and/or ANCOVAs, but using a MANOVA allows you to test for interactions between both independent and response variables all within one test, so is generally more reliable (though also more complicated).
I gave a presentation on MANOVA for Dyer's Ecological Analysis class last semester. You can find my presentation here, though it's a little messed up and geared towards SAS: http://www.tulane.edu/~ldyer/classes/396/nicole_files/frame.htm
I also found this webpage to be helpful when I was preparing my talk: http://online.sfsu.edu/~efc/classes/biol710/manova/manovanew.htm
Good luck!
Nicole
Hey Nicole,
After learning what MANOVA is, I realize that this is not what I would want to do at all. Thanks for the website link!
Post a Comment